Discover pathogen and host proteomes simulataneously
Using mass spectrometry-based proteomics, we took an unbiased, highly sensitive approach to profile both cellular proteomes and extracellular environments. With recent advances in technology and bioinformatics, we were able to simultaneously identify both host and pathogen components in a single experiment, providing an unprecedented, in-depth look into infection dynamics.
Focusing on the ocular surface, we explored how Pseudomonas aeruginosa contributes to biofilm formation, inflammation, and ocular tissue damage. Our study revealed key immune markers, including neutrophil proteins, which helped to identify biomarkers of infection and provided new insights into immune cell recruitment at the infection site. These biomarkers have potential for advancing diagnostic approaches for P. aeruginosa-associated keratitis.
Additionally, we mapped the most comprehensive murine corneal proteome to date, uncovering crucial proteins and networks involved in the host immune response. For the first time, we identified bacterial proteins attached to the ocular surface. Our findings were further validated through in silico comparisons and enzymatic profiling. This research offers a deep dive into the host-pathogen interface, revealing significant differences between general and site-specific immune responses, with important implications for improving diagnostics and treatments.
This work was an exceptional collaboration with Dr. Geddes-McAlister's team. Our publication, "Label-free proteomics distinguished general- and site-specific host responses to P. aeruginosa infection at the ocular surface," was published in Proteomics. Check out the link below!