Measuring complement levels and activation for biomarker development
This unit provides detailed protocols for measuring mannan-binding lectin (MBL) levels and MBL pathway activity in human plasma or serum, offering valuable tools for biomarker discovery and clinical research. By leveraging a modified ELISA approach, these assays utilize Europium (Eu³⁺)-labeled detection reagents instead of traditional enzyme-based methods, enhancing sensitivity and precision. The protocols include the use of anti-MBL antibodies or mannan-coated surfaces to detect MBL and assess its functional activity. Additionally, the unit features an MBL/MASP complex activity assay, enabling the quantification of MBL pathway-specific activity in biological samples. These assays provide a robust framework for evaluating MBL as a biomarker, facilitating translational research and diagnostic advancements in immune system disorders.
This work was published in Current Protocols. Explore the protocol using the link below!